Related Experiment Video
Updated: Aug 18, 2026

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
Published on: October 11, 2019
[Simultaneous determination of metronidazolum and vitamin B6 by the derivative spectrophotometry]
Abstract:
A new method for simultaneous determination of metronidazolum and vitamin B6 was established by the third derivative spectrophotometry in 0.1 mol/L HCl solution. The detection limits were 0.81 microg/mL for metronidazolum and 0.16 microg/mL for vitamin B6. The linear range of determination was 1.6-22.4 microg/mL for both metronidazolum and vitamin B6. This method has been applied to the determination of metronidazolum and vitamin B6 in tabellae metronidazoli compositae with satisfactory results. The coefficients of variation are below 2.26 and 2.65% and the recoveries are 93-102% and 99-101% for metronidazolum and vitamin 6, respectively.
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
UV–Vis Spectrometers
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
Complexometric Titration: Overview
