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Chemotherapy of an experimental Fusobacterium (Sphaerophorus) necrophorum infection in mice
Abstract:
An experimental animal model for testing antibiotics in vivo against Fusobacterium (Sphaerophorus) necrophorum has been developed. It incorporates the subcutaneous injection of the bacteria into mice followed by intraperitoneal administration of the antibiotic at 24, 48, 72, and 96 h. Mean effective dose values are based on the number of survivors 21 days after challenge. Tetracycline was the most effective drug tested, with a mean effective dose of 5.0 mg/kg, compared with mean effective dose values of 11.1 for clindamycin, 11.8 for penicillin-G, and 52.9 for lincomycin.
Insights
A new mouse model effectively tests antibiotics against Fusobacterium necrophorum infections. Tetracycline demonstrated superior efficacy compared to clindamycin, penicillin-G, and lincomycin in this in vivo study.
Area of Science:
- Veterinary Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Fusobacterium necrophorum is a significant pathogen in animal infections.
- Effective in vivo models are crucial for evaluating antibiotic efficacy.
- Limited data exists on comparative antibiotic effectiveness against F. necrophorum.
Purpose of the Study:
- To develop and validate an experimental animal model for testing antibiotics against Fusobacterium necrophorum.
- To compare the in vivo efficacy of tetracycline, clindamycin, penicillin-G, and lincomycin.
Main Methods:
- Subcutaneous injection of F. necrophorum into mice.
- Intraperitoneal administration of antibiotics at 24, 48, 72, and 96 hours post-infection.
- Assessment of survival rates at 21 days to determine Mean Effective Dose (MED).
Main Results:
- Tetracycline exhibited the highest efficacy with a MED of 5.0 mg/kg.
- Clindamycin (MED 11.1 mg/kg) and penicillin-G (MED 11.8 mg/kg) showed moderate effectiveness.
- Lincomycin displayed the lowest efficacy with a MED of 52.9 mg/kg.
Conclusions:
- The developed mouse model is suitable for in vivo antibiotic testing against F. necrophorum.
- Tetracycline is the most effective antibiotic among those tested for F. necrophorum infections in this model.
- Findings provide valuable data for selecting appropriate antibiotic treatments for F. necrophorum related diseases.
