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A virus-based nanoblock with tunable electrostatic properties.

Anju Chatterji1, Wendy F Ochoa, Takafumi Ueno

  • 1Department of Molecular Biology and Center for Integrative and Molecular Biosciences, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California, USA.

Nano Letters
|April 14, 2005
PubMed
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Cowpea mosaic virus was genetically modified with histidine tags, enabling controlled nickel binding and electrostatic properties. These HIS tags allowed for specific targeting and modification, demonstrating their utility in virus research.

Area of Science:

  • Virology
  • Biochemistry
  • Materials Science

Background:

  • Cowpea mosaic virus (CPMV) is a plant virus with a protein capsid.
  • Genetic engineering allows for modification of viral structures.
  • Histidine tags are commonly used for protein purification and manipulation.

Purpose of the Study:

  • To create HIS tag mutants of cowpea mosaic virus.
  • To investigate the binding properties and electrostatic characteristics of these mutants.
  • To demonstrate the specific addressability and modification of the HIS tag.

Main Methods:

  • Genetic engineering to introduce six contiguous histidine residues at various locations on the CPMV capsid.
  • Assessing differential affinity for nickel binding.

Related Experiment Videos

  • Analyzing electrostatic properties based on histidine protonation state.
  • Selective modification of HIS tags using nanogold conjugated to Ni-NTA.

    • Main Results:

    • Successfully generated five HIS tag mutants of CPMV.
    • Demonstrated differential nickel-binding affinities among mutants.
    • Showcased control over electrostatic properties via histidine protonation.
    • Confirmed specific HIS tag addressability through nanogold labeling.

    Conclusions:

    • HIS tag mutants of CPMV exhibit tunable nickel-binding and electrostatic properties.
    • The HIS tag provides a specific site for functionalization.
    • These engineered viruses offer potential for novel applications in nanotechnology and targeted delivery.