Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysing proteomic data.

J Barrett1, P M Brophy, J V Hamilton

  • 1Institute of Biological Sciences, University of Wales, Penglais, Aberystwyth, Ceredigion, Wales SY23 3DA, UK. jzb@aber.ac.uk

International Journal for Parasitology
|April 14, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mindfulness moderates the relationship between emotional eating and body mass index in a sample of people with cystic fibrosis.

Eating and weight disorders : EWD·2020
Same author

Correction to: The contribution of iron deficiency to the risk of peripartum transfusion: a retrospective case control study.

BMC pregnancy and childbirth·2020
Same author

The contribution of iron deficiency to the risk of peripartum transfusion: a retrospective case control study.

BMC pregnancy and childbirth·2020
Same author

Improved Upper Limit on the Neutrino Mass from a Direct Kinematic Method by KATRIN.

Physical review letters·2019
Same author

Mid-trimester cervical consistency index measurement and prediction of preterm birth before 34 and 37 weeks in twin pregnancy.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2019
Same author

Utility of ultrasound assessment in management of pregnancies with preterm prelabor rupture of membranes.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2019
Same journal

Hidden digenean diversity in two African protected areas: metabarcoding as a tool for parasite identification.

International journal for parasitology·2026
Same journal

Nearly Bitten, Twice Shy: Effects of prior and current parasite exposure on host mating behaviour.

International journal for parasitology·2026
Same journal

Bringing order to chaos: A new classification of avian schistosomes and their genetic lineages in the context of swimmer's itch.

International journal for parasitology·2026
Same journal

Assessing myxozoan diversity in fish: microscopy-based detection versus high-throughput amplicon sequencing.

International journal for parasitology·2026
Same journal

Spatial expression analysis and target prediction give insight into microRNA function in the model cestode Hymenolepis microstoma.

International journal for parasitology·2026
Same journal

Climatic suitability and phylogeography reveal cryptic diversity and public health risk in the Amblyomma testudinarium complex and other Asian Amblyomma.

International journal for parasitology·2026
See all related articles

Proteomics research relies on 2D gels and mass spectrometry for protein identification. New methods are needed for accurate gel alignment and cross-species protein identification, especially for organisms lacking sequenced genomes.

Area of Science:

  • Proteomics
  • Biochemistry
  • Bioinformatics

Background:

  • The growth of proteomics is driven by 2D gel electrophoresis and mass spectrometry.
  • 2D gels present challenges in reproducibility and require alignment for accurate spot matching.
  • Protein identification from gel spots necessitates unique identifying properties for database searching.

Purpose of the Study:

  • To explore methods for improving gel registration and protein identification in proteomics.
  • To address challenges in cross-species protein identification, particularly for organisms with unsequenced genomes.
  • To highlight the need for comprehensive metadata in proteomic database mining.

Main Methods:

  • Utilizing 2D gel electrophoresis and mass spectrometry for protein analysis.

Related Experiment Videos

  • Employing peptide mass fingerprinting and tandem mass spectrometry (MS/MS) for protein identification.
  • Investigating database searching strategies using molecular mass, pI, amino acid composition, and sequence tags.

    • Main Results:

    • Direct gel registration methods are preferable to indirect ones to preserve information.
    • Mass spectrometry, especially MS/MS, significantly enhances protein identification specificity.
    • Protein identification is challenging for organisms without sequenced genomes, limiting database search efficacy.

    Conclusions:

    • Improved reproducibility and direct registration methods are crucial for 2D gel-based proteomics.
    • Tandem mass spectrometry (MS/MS) is a powerful tool for specific protein identification.
    • Addressing the limitations of cross-species identification and the need for metadata is vital for future proteomic research.