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Related Experiment Videos

Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties.

K Yokogawa, S Kawata, S Nishimura

    Antimicrobial Agents and Chemotherapy
    |August 1, 1974
    PubMed
    Summary

    Mutanolysin, an enzyme from Streptomyces globisporus, effectively lyses bacteria linked to dental caries. Its stability in saliva suggests potential for caries control applications.

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    Area of Science:

    • Microbiology
    • Enzymology
    • Biochemistry

    Background:

    • Dental caries is a significant global health issue.
    • Streptococcus mutans and other oral bacteria are key etiologic agents of dental caries.
    • Enzymatic approaches for controlling cariogenic bacteria are of interest.

    Purpose of the Study:

    • To characterize mutanolysin, a lytic enzyme from Streptomyces globisporus.
    • To evaluate the lytic and bactericidal activity of mutanolysin against oral bacteria.
    • To assess the potential of mutanolysin for dental caries control.

    Main Methods:

    • Partial purification of mutanolysin from Streptomyces globisporus culture filtrate.
    • Determination of enzyme properties including isoelectric point, optimal pH, and temperature.

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  • Testing lytic and bactericidal activity against various bacterial strains, including Streptococcus mutans.
  • Assessing enzyme stability in human saliva and the effect of chemical agents.

    • Main Results:

    • Mutanolysin comprises two lytic enzymes and a associated proteolytic enzyme.
    • Optimal lytic activity was observed at 60°C and pH 6.5-7.0; enzyme stable at 50°C in acidic conditions.
    • Mutanolysin exhibited potent lytic/bactericidal activity against key dental caries pathogens (e.g., Streptococcus mutans, Lactobacillus acidophilus) but not against S. aureus or Gram-negative bacteria.
    • Lytic activity was retained in human saliva, and cell wall digestion released amino groups and reducing sugars.

    Conclusions:

    • Mutanolysin possesses specific lytic activity against cariogenic oral bacteria.
    • The enzyme's stability in saliva and its mechanism of action on bacterial cell walls support its potential.
    • Mutanolysin shows promise as a novel agent for controlling dental caries.