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Related Experiment Videos

[Study of solid-phase time-resolved fluorescence label immunoassay].

Li-hua Pan1, Si-hong Zhou, Wen-wei Sun

  • 1National Analytical Research Center of Electrochemistry and Spectroscopy, Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|April 15, 2005
PubMed
Summary

This study optimizes labeling of Hepatitis B surface antibody IgG (HBsAbIgG) using a novel fluorescence probe (BCPDA) for time-resolved fluorimmunoassays. The method ensures high bioactivity and stable, intense fluorescence for improved diagnostics.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Immunodiagnostics

Context:

  • Solid-phase time-resolved fluorimmunoassays (TRFIA) are crucial for sensitive detection.
  • Hepatitis B surface antibody IgG (HBsAbIgG) detection is vital for diagnosing Hepatitis B virus infections.
  • Developing efficient labeling methods is key to enhancing immunoassay performance.

Purpose:

  • To determine optimal conditions for labeling HBsAbIgG with 4,7-bis-chorosulfophenyl-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA).
  • To characterize the properties of the resulting protein-fluorophore complex for TRFIA applications.

Summary:

  • BCPDA reacts with HBsAbIgG under mild conditions, preserving over 80% bioactivity.
  • Optimal BCPDA to HBsAbIgG molar ratio is 45-70, with high recovery (>80%).

Related Experiment Videos

  • The stable Protein-BCPDA-Eu3+ complex exhibits high fluorescence intensity, long lifetimes, a large Stokes shift (270 nm), and a narrow emission band (611.2 nm).

    • Impact:

    • Provides a foundation for developing non-isotopic immunoassay techniques.
    • Facilitates advancements in clinical medical diagnosis for Hepatitis B.
    • Offers a highly sensitive and stable fluorescent labeling strategy for biomolecules.