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Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman
Gajendra P Singh1, Caitriona M Creely, Giovanni Volpe
1ICFO-Institut de Ciències Fotoniques, Jordi Girona 29, Nexus II, 08034 Barcelona, Spain.
Analytical Chemistry
|April 15, 2005
Abstract:
Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell.
