Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput SNP genotyping on universal bead arrays.

Richard Shen1, Jian-Bing Fan, Derek Campbell

  • 1Illumina Inc., 9885 Towne Centre Drive, San Diego, CA 92121, USA. rshen@illumina.com

Mutation Research
|April 15, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expanding the Clinical and Genetic Landscape of UQCRC2-related Mitochondrial Complex III Deficiency: A Case Report and Literature Review.

Current pediatric reviews·2026
Same author

Screening for Cervical Cancer: A Recommendation From the Women's Preventive Services Initiative.

Obstetrics and gynecology·2026
Same author

Comparison of clinical-radiological and radiomics features for predicting pulmonary nodule malignancy in a multicenter study of mixed clinical and surveillance populations.

European radiology·2026
Same author

Re: Self-Collected Vaginal Specimens at Home for HPV Testing: Recommendations From the Enduring Guidelines Committee.

Journal of lower genital tract disease·2026
Same author

Poor efficacy of anti PD-1 antibody based immunotherapy in patients with acral melanoma: results from the Spanish Melanoma Group (GEM) registry.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2025
Same author

Recommendations From the Women's Preventive Services Initiative on Breast Cancer Screening for Women at Average Risk and Patient Navigation Services for Breast and Cervical Cancer Screening.

Obstetrics and gynecology·2025

A new SNP genotyping assay offers flexible, accurate, and high-throughput genetic analysis for large populations. This system enables rapid generation of millions of genotypes, making genetic research more accessible and efficient.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • High-throughput genetic analysis is crucial for understanding population genetics and disease.
  • Existing SNP genotyping methods can be limited in multiplexing capacity and throughput.
  • There is a need for scalable and cost-effective genotyping solutions for large-scale studies.

Purpose of the Study:

  • To develop a flexible, accurate, and highly multiplexed SNP genotyping assay.
  • To enable high-throughput genetic analysis of large populations using a bead array platform.
  • To enhance the efficiency and affordability of genetic research.

Main Methods:

  • Development of a novel genotyping system linking SNP oligos to unique address sequences.
  • Utilizing bead arrays on optical fiber bundles (Sentrix Array Matrix) or silicon slides (Sentrix BeadChip).

Related Experiment Videos

  • Implementation of Array of Arrays formats for parallel sample processing.

    • Main Results:

    • Achieved high assay conversion rates and data quality with >1500 multiplexing.
    • Demonstrated parallel processing capabilities with Sentrix Array Matrix and BeadChip formats.
    • Enabled generation of approximately 300,000 genotypes per day (minimal equipment) to >1.6 million genotypes per day (robotics-assisted).

    Conclusions:

    • The developed SNP genotyping assay provides a flexible, accurate, and high-throughput solution for genetic research.
    • The system significantly increases the number of genotypes that can be generated daily.
    • This technology offers a new level of throughput and affordability for large-scale genetic studies.