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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

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Analysis of recombination signal sequences in zebrafish

Nadia Danilova1

  • 1Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. ndanilova@mit.edu

Molecular Immunology
|April 15, 2005
PubMed

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...