Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phytoestrogen exposure elevates PTEN levels.

Kristin A Waite1, Michelle R Sinden, Charis Eng

  • 1Clinical Cancer Genetics Program, Comprehensive Cancer Center, Division of Human Genetics, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA.

Human Molecular Genetics
|April 15, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Embryonic stem cell factor FOXD3 (Genesis) defects in gastrointestinal stromal tumors.

Endocrine-related cancer·2023
Same author

A Randomized Trial Comparing the Effectiveness of Pre-test Genetic Counseling Using an Artificial Intelligence Automated Chatbot and Traditional In-person Genetic Counseling in Women Newly Diagnosed with Breast Cancer.

Annals of surgical oncology·2023
Same author

Development of webcam-collected and artificial-intelligence-derived social and cognitive performance measures for neurodevelopmental genetic syndromes.

American journal of medical genetics. Part C, Seminars in medical genetics·2023
Same author

Hemizygous variants in protein phosphatase 1 regulatory subunit 3F (PPP1R3F) are associated with a neurodevelopmental disorder characterized by developmental delay, intellectual disability and autistic features.

Human molecular genetics·2023
Same author

Tamoxifen and the risk of breast cancer in women with a BRCA1 or BRCA2 mutation.

Breast cancer research and treatment·2023
Same author

Integrating somatic CNV and gene expression in breast cancers from women with PTEN hamartoma tumor syndrome.

NPJ genomic medicine·2023
Same journal

Circulating MYOM3 fragments reflect disease severity and therapeutic efficacy in tubular aggregate myopathy and Stormorken syndrome.

Human molecular genetics·2026
Same journal

The FVB-nmd SMARD1 mouse presents with early respiratory deficits and pathology that significantly impact lifespan.

Human molecular genetics·2026
Same journal

Utrophin requires α-Syntrophin to maintain neuromuscular junction integrity in mdx mice.

Human molecular genetics·2026
Same journal

A novel gene ACTRT3 mutations induce sperm malformations and fertilization failure via Acrosomal ultrastructural defects.

Human molecular genetics·2026
Same journal

Nucleic acid-based therapeutic strategies for modulator-refractory cystic fibrosis-causing variants.

Human molecular genetics·2026
Same journal

Evidence that disruption of Discoidin domain receptor 2 contributes to palate malformations through effects on the extracellular matrix.

Human molecular genetics·2026
See all related articles

Phytoestrogens, found in certain foods, may help prevent breast cancer by increasing PTEN protein levels. This supports the role of diet in cancer prevention and therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Nutritional Science

Background:

  • Phytoestrogens are plant compounds with potential protective effects against breast cancer.
  • PTEN (Phosphatase and Tensin homolog) is a critical tumor suppressor involved in cell division, and its mutations are common in breast cancer.
  • Active PTEN regulates cell proliferation and apoptosis by influencing key signaling pathways like Akt and MAPK.

Purpose of the Study:

  • To investigate whether phytoestrogen exposure influences PTEN protein expression in the MCF-7 breast cancer cell line.
  • To explore the downstream effects of phytoestrogen-induced PTEN regulation on cell signaling pathways.
  • To elucidate the transcriptional mechanism underlying phytoestrogen-mediated changes in PTEN expression.

Main Methods:

Related Experiment Videos

  • MCF-7 breast cancer cells were treated with phytoestrogens (resveratrol, quercetin, genistein).
  • Protein levels of PTEN, phosphorylated Akt, p27, phosphorylated MAPK, and cyclin D1 were analyzed.
  • Semi-quantitative RT-PCR was used to assess PTEN mRNA levels.

    • Main Results:

    • Phytoestrogen stimulation led to increased PTEN protein levels in MCF-7 cells.
    • This was accompanied by decreased Akt phosphorylation and increased p27 levels, indicating enhanced PTEN lipid phosphatase activity.
    • PTEN's protein phosphatase activity, assessed via MAPK phosphorylation and cyclin D1 levels, remained unchanged. PTEN mRNA levels showed a slight increase, suggesting transcriptional regulation.

    Conclusions:

    • Phytoestrogen exposure increases PTEN protein expression in breast cancer cells, likely via transcriptional regulation.
    • This mechanism contributes to the potential anti-cancer effects of phytoestrogens.
    • Targeting PTEN expression through dietary phytoestrogens may offer adjunctive strategies for breast cancer prevention and therapy.