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Short communication: Ca2+-adenosine triphosphatase protein expression in the mammary gland of periparturient cows
S Prapong1, T A Reinhardt, J P Goff
1Periparturient Diseases of Cattle Research Unit, USDA-ARS, National Animal Disease Center, Ames, IA 50010, USA.
The objectives of this study were to measure the changes in protein expression of the mammary Ca2+-ATPases during the periparturient period and to determine whether Ca2+-ATPase protein expression in the mammary gland is related to milk fever (MF) development. Abundance of Ca2+-ATPase in mammary tissue and milk fat globule membranes was determined by Western blotting. The secretory pathway Ca2+-ATPase was elevated prepartum in mammary tissue from cows that developed MF compared with non-MF cows.
The objectives of this study were to measure the changes in protein expression of the mammary Ca2+-ATPases during the periparturient period and to determine whether Ca2+-ATPase protein expression in the mammary gland is related to milk fever (MF) development. Abundance of Ca2+-ATPase in mammary tissue and milk fat globule membranes was determined by Western blotting. The secretory pathway Ca2+-ATPase was elevated prepartum in mammary tissue from cows that developed MF compared with non-MF cows.

