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Mapping quantitative trait loci associated with leaf and stem pubescence in cotton
1CIRAD (UMR 1096), TA 70/03, Avenue Agropolis, 34398 Montpellier Cedex 5, France. marc.lacape@cirad.fr
The Journal of Heredity
|April 15, 2005
Summary
Leaf pubescence in cotton, controlled by genes like t(1)-t(5), can aid insect pest management. Nine quantitative trait loci (QTLs) were identified, with chromosome 6 being a major region for leaf pubescence.
Area of Science:
- Genetics
- Agronomy
- Plant Breeding
Background:
- Leaf pubescence in cotton (Gossypium species) is a trait with potential applications in insect pest management.
- Previous research has associated varying leaf trichome densities with five genes, designated t(1) through t(5).
Purpose of the Study:
- To qualitatively and quantitatively assess leaf and stem pubescence in cotton.
- To identify quantitative trait loci (QTLs) associated with leaf pubescence using interspecific backcross populations.
Main Methods:
- Utilized two segregating interspecific Gossypium hirsutum x G. barbadense backcross populations.
- Performed quantitative trait loci (QTL) analyses using both simple and composite interval mapping.
- Assessed leaf and stem pubescence both qualitatively and quantitatively.
Main Results:
- Identified nine significant QTLs for leaf pubescence, mapping to four distinct chromosomal regions.
- Chromosome 6 (c6) and chromosome D03 showed the highest LOD values, with G. hirsutum alleles increasing pubescence.
- QTLs on chromosome 17 (c17) and chromosome A01 indicated that G. hirsutum alleles negatively impacted pubescence.
Conclusions:
- Confirmed the t(1) locus on chromosome 6 as a major determinant of leaf pubescence.
- Identified additional genes on seven other chromosomes that influence trichome density, either positively or negatively.
- High-density PCR-based loci in identified QTL regions, especially on chromosome 6, will aid map-based cloning efforts.