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Updated: Aug 18, 2026
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
[Molecular cloning of a protein antigen gene of Cysticercus cellulosae]
Dian-wu Liu1, Wen-ling Li, Li-mei Zhang
1Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017, China.
Objective:
To immunoscreen one protein antigen gene from a cDNA library of Cysticercus cellulosae.
Methods:
A eDNA library of C. cellulosae was constructed after cDNA was synthesized, and immunoscreened using rabbit anti-C. cellulosae polyclone antibody. The gene structure and its possible function were analyzed by comparing with the sequences available in the GenBank, after the insert of positive clone was subcloned to pBluescript SK plasmid and the nucleotide sequence of the cDNA was determined by dideoxynucleotide chain termination method using a Taq DyeDeoxy Terminator Cycle Sequencing Kit. The amino acid sequence was deduced from nucleotide sequence using GENETYX software. Homological search of the nucleotide sequences was done using BLAST in GenBank.
Results And Conclusion:
A cDNA clone of 1320 bp was isolated. The clone contained one open reading frame composed of 1260 bp encoding 420 amino acids, in which two potential glycosylation sites were found. The partial nucleotide sequence of the gene fragment showed high homology with the essential spectrin gene of Caenorhabditis elegans and the erythrocyte surface antigen gene of Plasmodium falciparum, when the gene fragment was homologically analyzed in GenBank.
