Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-speed digital imaging method for ciliary beat frequency measurement.

Svetlana Dimova1, Frederik Maes, Marcus E Brewster

  • 1Johnson and Johnson, Pharmaceutical Research and Development, A Division of Janssen Pharmaceutica NV, Beerse, Belgium.

The Journal of Pharmacy and Pharmacology
|April 16, 2005
PubMed
Summary

A new high-speed digital imaging system offers a faster, automated, and reliable method for analyzing ciliary beat frequency (CBF). This observer-independent system provides results interchangeable with traditional methods, improving efficiency in research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated Classification of Neuromuscular Diseases Using Thigh Muscle MRI With Model Interpretations.

Journal of cachexia, sarcopenia and muscle·2025
Same author

Artificial Intelligence (AI)-assisted readout method for the evaluation of skin prick automated test results.

Nature communications·2025
Same author

Automated quantification of lung pathology on micro-CT in diverse disease models using deep learning.

EBioMedicine·2025
Same author

Local response function estimation in spherical deconvolution for comprehensive tissue representation using diffusion MRI.

Imaging neuroscience (Cambridge, Mass.)·2025
Same author

Long-term outcomes of endoscopic resection and tailored adjuvant radiotherapy for sinonasal intestinal-type adenocarcinoma: a historical single-center cohort study in 200 patients.

Frontiers in oncology·2025
Same author

Real-time automated assessment of histological disease activity in patients with ulcerative colitis using single-wavelength endoscopy technology.

Endoscopy·2025

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Respiratory Physiology

Background:

  • Ciliary beat frequency (CBF) analysis is crucial for understanding mucociliary clearance.
  • Conventional methods like computerized microscope photometry are time-consuming and observer-dependent.
  • There is a need for automated, faster, and reliable CBF measurement techniques.

Purpose of the Study:

  • To develop and validate a high-speed digital imaging system and software for automated CBF analysis.
  • To establish an observer-independent and efficient alternative to existing photometry methods.
  • To compare the performance of the new system with conventional techniques.

Main Methods:

  • Development of a high-speed digital imaging system and associated analysis software.

Related Experiment Videos

  • Utilized primary human nasal epithelial cell cultures for experiments.
  • Employed glycocholate and chlorocresol as ciliostimulatory and cilio-inhibitory agents.
  • Compared measurements with a conventional computerized microscope photometry system.

    • Main Results:

    • The high-speed digital imaging system yielded CBF values comparable to the photometry method.
    • Both systems showed similar percentage changes in CBF upon stimulation (glycocholate) and inhibition (chlorocresol).
    • Bland-Altman analysis indicated that the results from both methods are interchangeable (mean difference 0.2 +/- 0.6 Hz).

    Conclusions:

    • The developed high-speed digital imaging system is reliable for in-vitro CBF measurements.
    • The system offers advantages such as rapid data acquisition, automated analysis, and reduced selection bias.
    • This technology provides a more efficient and objective approach to CBF analysis.