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Differential expression of L-selectin ligand in the endometrium during the menstrual cycle
Tsung-Hsuan Lai1, Ie-Ming Shih, Nikos Vlahos
1Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Objective:
To evaluate the expression of L-selectin ligand in the endometrium during the menstrual cycle.
Design:
Retrospective study.
Setting:
University teaching hospital.
Patient(S):
Endometrial samples from regularly cycling women.
Intervention(S):
Tissue microarray and immunohistochemical staining were performed using L-selectin ligand monoclonal antibody (MECA-79).
Main Outcome Measure(S):
Expression of L-selectin ligand in the various phases of menstrual cycle was measured by a semiquantitative analysis (HSCORE) for the intensity of immunohistochemical reactivity.
Result(S):
In the luminal epithelium, there were significant differences in L-selectin ligand expression during the proliferative, interval, early secretory, and midsecretory phases. The expression of L-selectin ligand was greatest from the periovulatory interval through midsecretory phase. In the glandular epithelium, the expression of L-selectin ligand was greatest in midsecretory phase with significant differences between proliferative phase and the midsecretory phase and between the interval phase and the midsecretory phase.
Conclusion(S):
Increased expression of L-selectin ligand in the human endometrium during the early and midsecretory phases of the menstrual cycle may be related to the process of implantation.
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