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Related Experiment Videos

A trial to restore defective human sperm centrosomal function.

Soichi Nakamura1, Yukihiro Terada, Vanesa Y Rawe

  • 1Department of Obstetrics and Gynecology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan.

Human Reproduction (Oxford, England)
|April 16, 2005
PubMed
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Treatments with dithiothreitol (DTT) and paclitaxel can restore sperm centrosome function in some cases. However, this method is ineffective for dysplasia of the fibrous sheath (DFS) sperm, indicating severe centrosome dysfunction.

Area of Science:

  • Reproductive biology
  • Cell biology
  • In vitro fertilization

Background:

  • Sperm centrosome function is critical for successful human fertilization, enabling pronuclear development and fusion.
  • Abnormalities in sperm microtubule organization can impair fertilization outcomes.
  • Investigating methods to restore centrosome function in defective sperm is essential for improving fertility treatments.

Purpose of the Study:

  • To explore the potential of chemical treatments to restore centrosomal function in human sperm with microtubule organization defects.
  • To evaluate the efficacy of dithiothreitol (DTT) and paclitaxel in re-establishing sperm centrosome function.

Main Methods:

  • Semen samples were obtained from a fertile donor and a patient with dysplasia of the fibrous sheath (DFS).

Related Experiment Videos

  • Heterologous intracytoplasmic sperm injection (ICSI) was performed using treated sperm and bovine oocytes.
  • Sperm were pre-treated with DTT, and oocytes were post-treated with paclitaxel.

    • Main Results:

    • Combined DTT and paclitaxel treatment successfully induced microtubule organization in dead fertile donor sperm after ICSI.
    • The DTT and paclitaxel treatment was ineffective in restoring function to DFS sperm.
    • DFS sperm showed reduced expression of centrin, a key centrosomal protein, compared to fertile donor sperm.

    Conclusions:

    • Sperm centrosome function can be induced via pre-ICSI DTT treatment and post-ICSI paclitaxel treatment.
    • DFS sperm exhibit severe centrosome dysfunction that is not overcome by these treatments.
    • This treatment approach may serve as a diagnostic tool to assess the severity of sperm centrosomal dysfunction.