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Related Experiment Videos

Interactions between live Bartonella bacilliformis and endothelial cells.

F U Garcia1, J Wojta, R L Hoover

  • 1Pathology Department, Vanderbilt University, Nashville, Tennessee.

The Journal of Infectious Diseases
|June 1, 1992
PubMed
Summary
This summary is machine-generated.

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Live Bartonella bacilliformis bacteria stimulate endothelial cell proliferation and tissue-type plasminogen activator (t-PA) production, forming Rocha-Lima inclusions. These findings support the bacterium's role in the vascular proliferation of verruca peruana.

Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogenesis

Background:

  • Bartonella bacilliformis causes verruca peruana, forming Rocha-Lima inclusions in endothelial cells.
  • Previous studies showed B. bacilliformis homogenates promote endothelial cell proliferation and angiogenesis.

Purpose of the Study:

  • To investigate if live B. bacilliformis stimulates endothelial cells and forms Rocha-Lima inclusions.
  • To confirm the angiogenic activity of live B. bacilliformis.

Main Methods:

  • In vitro assays measuring endothelial cell proliferation and tissue-type plasminogen activator (t-PA) production.
  • Electron microscopy to observe bacterial interaction with endothelial cells and inclusion formation.

Main Results:

Related Experiment Videos

  • Live B. bacilliformis significantly increased endothelial cell proliferation and t-PA production, similar to bacterial homogenates.
  • Bacteria penetrated endothelial cells within 1 hour, forming membrane-bound inclusions.
  • Large Rocha-Lima-like inclusions containing numerous bacteria developed within 12 hours.

    • Conclusions:

    • Live B. bacilliformis possesses angiogenic activity.
    • The bacteria are directly responsible for the vascular proliferation observed in verruca peruana.
    • Bacterial interaction with endothelial cells is crucial for proliferation and inclusion formation.