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Related Experiment Videos

C5L2 is a functional receptor for acylation-stimulating protein.

David Kalant1, Robin MacLaren, Wei Cui

  • 1Mike Rosenbloom Laboratory for Cardiovascular Research, Division of Cardiology, Department of Medicine, McGill University Health Centre, Montreal, Quebec H3A 1A1, Canada.

The Journal of Biological Chemistry
|April 19, 2005
PubMed
Summary

The C5L2 receptor mediates acylation-stimulating protein (ASP) effects on triglyceride synthesis and glucose transport. This study demonstrates C5L2

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolism

Background:

  • C5L2 is known to bind acylation-stimulating protein (ASP) with high affinity.
  • The precise functionality of C5L2 in cellular processes remained uncharacterized.
  • ASP plays a role in adipocyte differentiation and lipid metabolism.

Purpose of the Study:

  • To elucidate the functional role of C5L2 in ASP-mediated cellular responses.
  • To investigate C5L2 expression in human and mouse adipose tissues.
  • To determine the molecular mechanism by which C5L2 mediates ASP signaling.

Main Methods:

  • Stable transfection of human C5L2 cDNA into HEK293 cells.
  • Assessment of triglyceride synthesis and glucose transport in response to ASP.

Related Experiment Videos

  • Antisense oligonucleotide-mediated down-regulation of C5L2 in human skin fibroblasts and 3T3-L1 preadipocytes.
  • Analysis of beta-arrestin translocation and C5L2 phosphorylation in HEK-hC5L2 cells.

    • Main Results:

    • C5L2 expression was confirmed in human and mouse adipose tissues.
    • ASP stimulation of triglyceride synthesis and glucose transport was observed in C5L2-expressing cells.
    • Down-regulation of C5L2 led to a significant loss of ASP responsiveness.
    • ASP induced beta-arrestin translocation and C5L2 phosphorylation, indicating receptor activation.

    Conclusions:

    • C5L2 functions as a receptor mediating ASP-stimulated triglyceride synthesis.
    • This study provides the first demonstration of C5L2's functional role in cellular signaling.
    • C5L2 is a key mediator of ASP's metabolic effects in adipocytes.