Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optical micromanipulations inside yeast cells.

Leonardo Sacconi1, Iva M Tolić-Nørrelykke, Chiara Stringari

  • 1University of Trento, 38050 Povo (Trento), Italy. sacconi@lens.unifi.it

Applied Optics
|April 20, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OPA1 Deficiency Impairs NGF Signaling and Drives Sympathetic Neurodegeneration.

JACC. Basic to translational science·2026
Same author

Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.

Biomedical optics express·2026
Same author

In vivo autofluorescence lifetime imaging of spatial metabolic heterogeneities and learning-induced changes in the <i>Drosophila</i> mushroom body.

eLife·2026
Same author

The cardiac neurovascular unit: sympathetic control of the capillary network in aging and transplantation.

American journal of physiology. Cell physiology·2026
Same author

A Label-Free Hyperspectral Imaging Device for Ex Vivo Characterization and Grading of Meningioma Tissues.

Journal of biophotonics·2025
Same author

Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.

Communications biology·2025
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

This study combines nonlinear microscopy and optical trapping to image and move structures inside living cells, offering a new tool for cell biology research.

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy
  • Optical Tweezers

Background:

  • Precise manipulation of intracellular structures is crucial for understanding cell function.
  • Existing methods for organelle manipulation can be inefficient or disadvantageous.
  • Nonlinear microscopy offers high-resolution imaging of cellular components.

Purpose of the Study:

  • To develop and demonstrate a combined nonlinear microscopy and optical trapping technique.
  • To enable three-dimensional imaging and manipulation of intracellular structures in living cells.
  • To provide an alternative method for organelle manipulation, such as nuclear positioning.

Main Methods:

  • Utilized Titanium-sapphire laser pulses for nonlinear microscopy of nuclear envelope and microtubules.

Related Experiment Videos

  • Employed optical trapping to capture and manipulate endogenous lipid granules within fission yeast cells.
  • Used trapped granules as handles to indirectly push and displace the cell nucleus.

    • Main Results:

    • Successfully performed simultaneous nonlinear microscopy and optical manipulation without significant cell damage or heating.
    • Demonstrated indirect displacement of the cell nucleus using a trapped lipid granule.
    • Showcased the capability to image and manipulate intracellular components in three dimensions.

    Conclusions:

    • The combined technique is a valuable tool for cell biology, offering precise manipulation of specific structures.
    • This method serves as an alternative to genetic and biochemical approaches for studying cellular processes.
    • Potential applications include investigating nuclear positioning mechanisms and cell division coordination.