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[Effects of connective tissue growth factor antisense oligonucleotides on the cultured human keloid fibroblasts in
Jian-yi Liu1, Shi-rong Li, Shu-xing Ji
1The Department of Plastic and Aesthetic Surgery, the Southwest Hospital of the Third Military Medical University, Chongqing 400038, China.
Objective:
To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid.
Methods:
CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyanate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblast (HKF) culturing media. The intracellular distribution of CTGF ASODN was observed with fluorescence microscopy in the fixed HKF. The proliferation of HKF was measured by MIT test. The apoptosis of HKF was measured with a flow cytometer. The collagen synthesis of HKF was measured by using H3-proline incorporation method.
Results:
The CTGF ASODN inhibited the proliferation and collagen synthesis of the HKF, compared with the control, but it increased the apoptosis after the transfection (P < 0.01).
Conclusion:
CTGF ASODN may has anti-fibrotic effects on human keloid in vitro, and the CTGF may play an important role in promoting the fibrosis of human keloid.
Insights
Connective tissue growth factor (CTGF) antisense oligonucleotides (ASODN) were tested against human keloid fibroblasts. CTGF ASODN demonstrated anti-fibrotic effects by inhibiting proliferation and collagen synthesis while increasing apoptosis.
Area of Science:
- Dermatology
- Cell Biology
- Molecular Biology
Context:
- Keloids are abnormal scars resulting from excessive extracellular matrix deposition.
- Connective tissue growth factor (CTGF) is implicated in the pathogenesis of fibrotic conditions, including keloids.
Purpose:
- To investigate the role of CTGF in human keloid pathogenesis.
- To evaluate the anti-fibrotic potential of CTGF antisense oligonucleotides (ASODN) in human keloid fibroblasts (HKF).
Summary:
- CTGF ASODN were delivered into HKF using liposomes and observed via fluorescence microscopy.
- The study measured HKF proliferation (MIT assay), apoptosis (flow cytometry), and collagen synthesis (H3-proline incorporation).
- Results showed CTGF ASODN significantly inhibited HKF proliferation and collagen synthesis, while concurrently increasing apoptosis (P < 0.01).
Impact:
- CTGF ASODN exhibit potential anti-fibrotic properties against human keloids in vitro.
- These findings suggest CTGF plays a crucial role in promoting keloid fibrosis.
- This research may inform novel therapeutic strategies for keloid treatment.
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