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Updated: Aug 18, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Electrophoresis of ionic microgel particles: from charged hard spheres to polyelectrolyte-like behavior
A Fernández-Nieves1, M Márquez
1Group of Complex Fluids Physics, Department of Applied Physics, University of Almería, 04120 Almería, Spain.
Abstract:
We perform electrophoretic mobility measurements of ionic microgel particles in the deswollen and swollen phases. The results show that microgels behave as charged hard spheres in the first case and as free-draining spherical polyelectrolytes in the latter. A unified theory for the electrophoresis of polyelectrolyte-coated particles [H. Ohshima, Adv. Colloid Interface Sci. 62, 189 (1995)] is shown to contain the essential physics for describing the experiments, upon adequate consideration of the particles swelling behavior and network-solvent friction variations.
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