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Published on: October 18, 2010
Specific molecular detection of Carnobacterium piscicola SF668 in cold smoked salmon
E Pellé1, X Dousset, H Prévost
1Laboratoire de Microbiologie Alimentaire et Industrielle, ENITIAA, Nantes, France.
Aims:
To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS).
Methods And Results:
CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time intervals and analysed by counting the number of viable cells. About 25-100% of colonies grown on Elliker plates were subjected to mPCR amplification. The results show that strains presumably identified as C. piscicola SF668 were predominant over the test period.
Conclusions:
mPCR is a powerful tool to study competitiveness of C. piscicola SF668, which inhibits the growth of Listeria monocytogenes.
Significance And Impact Of The Study:
The present study demonstrates the importance of molecular methods in studying competitiveness of strains with potential food applications.
