[Distribution of Orexin-A mRNA expression in different organs and its variation in acute inflammation]
Guang-Tao Yan1, Ji Lin, Jie Liao
1Research Laboratory of Biochemistry, General Hospital of PLA, Beijing 100853, China. yan301@263.net
Objective:
To explore the distribution of Orexin-A mRNA expression in different organs, and its variation in acute inflammation, and to evaluate the role of Orexin-A in acute inflammatory responses.
Methods:
Hypothalamus, adipose tissue, lung, stomach, duodenum, liver, spleen and testis of male Sprague-Dawley rats (including normal and those of intestinal ischemia/reperfusion injury model) were harvested, and reverse transcription-polymerase chain reaction(RT-PCR) was used to assess Orexin-A mRNA expression in different tissues of normal rats, and that in the hypothalamus of ischemia/reperfusion injured rats.
Results:
All tissues expressed Orexin-A mRNA, and it was especially high in the stomach, lung and kidney. After the acute stimulus of ischemia/reperfusion injury, Orexin-A mRNA expression showed a trend of fluctuant declination in the hypothalamus.
Conclusion:
Orexin-A mRNA is widely expressed in the major organs, and it has a time-dependent response to acute inflammatory stimuli. The results suggest that its expression variation may be used as a novel marker for acute inflammation.
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