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Crystallization of the exo(1,3)-beta-glucanase from Candida albicans
S Cutfield1, G Brooke, P Sullivan
1Biochemistry Department, University of Otago, Dunedin, New Zealand.
Abstract:
An exoglucanase, with specificity for beta (1,3) linkages, from the cell wall of Candida albicans has been crystallized by the hanging drop method in the presence of polyethylene glycol 8000. The crystals, which diffract to better than 1.9 A resolution, belong to the orthorhombic space group P212121 with cell constants a = 60.2 A, b = 65.2 A, c = 96.5 A and with one molecule in the asymmetric unit.
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