N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
Zhi-Qiang Wang1, Jinfang Liao, Zhenjun Diwu
1Department of Reagent and Assay Development, Molecular Devices Corporation, 1311 Orleans Drive, Sunnyvale, CA 94089, USA. zhiqiang_wang@moldev.com
Bioorganic & Medicinal Chemistry Letters
|April 20, 2005
Abstract:
A novel caspase-3 substrate N-Ac-DEVD-N'-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.
