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[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay]
Lei Wang1, Ying-Song Wu, Yong-Ping Tang
1Institute of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Objective:
To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA).
Methods:
Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit.
Result:
The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%;-6.2%;, and coefficient of variability between days of 5.3%;-9.6%;. The results of detection were consistent between ELISA and TRFIA.
Conclusion:
TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.
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