[High-performance liquid chromatography combined with mass spectrum analysis for chromatographic fingerprinting of
Yuan Yan1, Shi-Ting Liu, Zhong-Yuan Xu
1Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases.
Methods:
Three-dimensional high-performance liquid chromatography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting.
Result:
By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde.
Conclusion:
HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
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