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Published on: November 1, 2018
[Effects of Alternanthera philoxeroides Griseb against dengue virus in vitro]
Wen-Ling Jiang1, Xian-Ling Luo, Su-Juan Kuang
1Medical Research Center of People's Hospital of Guangdong Province, Guangzhou 510080, China. jwenling@126.com
Objective:
To investigate the effects of Alternanthera philoxeroides Griseb extracts against dengue virus in vitro.
Methods:
MTT assay and observation of cytopathic effect (CPE) were carried out to determine the cytotoxicity of Alternanthera philoxeroides Griseb on C6/36 cell lines and its effects on dengue virus.
Results:
None of the 4 kinds of Alternanthera philoxeroides Griseb extracts exhibited obvious cytotoxicity on the cells at different concentrations with the exception of that over 320 microg/ml. The 4 extracts all showed inhibitory effects on dengue virus. Statistical analysis of TD(50) and ED(50) by Probit regression method suggested that extracts from coumarin had the lowest toxicity on the cells (TD(50)=535.91), whereas extracts from petroleum ether showed the strongest inhibitory effects on dengue virus (ED(50)=47.43) among the 4 extracts.
Conclusion:
Alternanthera philoxeroides Griseb possesses antiviral effects on dengue virus in vitro.
Insights
Alternanthera philoxeroides Griseb extracts show antiviral activity against dengue virus in laboratory studies. These plant extracts demonstrated inhibitory effects on the virus without significant toxicity to cells.
Area of Science:
- Phytochemistry
- Virology
- Cell Biology
Context:
- Dengue virus poses a significant global health threat, necessitating novel therapeutic strategies.
- Natural products, such as plant extracts, are increasingly explored for antiviral properties.
Purpose:
- To evaluate the in vitro antiviral efficacy of Alternanthera philoxeroides Griseb extracts against dengue virus.
- To assess the cytotoxicity of these extracts on C6/36 cell lines.
Summary:
- Four types of Alternanthera philoxeroides Griseb extracts were tested. None exhibited significant cytotoxicity below 320 microg/ml.
- All extracts demonstrated inhibitory effects on dengue virus replication in vitro.
- Petroleum ether extracts showed the most potent antiviral activity (ED(50)=47.43), while coumarin extracts had the lowest cellular toxicity (TD(50)=535.91).
Impact:
- This study supports the potential of Alternanthera philoxeroides Griseb as a source of antiviral compounds against dengue virus.
- Findings provide a basis for further research into the development of phytomedicines for dengue treatment.
