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Updated: Aug 18, 2026
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Published on: April 21, 2022
Quantitative evaluation of ischemia-reperfusion injury by optical coherence tomography in the rat retina
Kenichiro Sho1, Kanji Takahashi, Toshio Fukuchi
1Department of Ophthalmology, Kansai Medical University, Osaka, Japan. shouk@takii.kmu.ac.jp
Purpose:
To determine whether optical coherence tomography (OCT) can detect early retinal changes after ischemia-reperfusion injury in rats.
Methods:
The intraocular pressure (IOP) was elevated to induce retinal ischemia in brown Norway rats. After 90 min of ischemia, the IOP was reduced, and after reperfusion of 1, 2, 4, or 7 days, OCT was performed. After the OCT examination, the eyes were enucleated and histological sections were made.
Results:
The OCT-determined mean retinal thickness was 168 +/- 16.9 microm in the untreated control group, and 177 +/- 2.16, 170 +/- 7.55, 159 +/- 5.34, and 140 +/- 5.56 microm on days 1, 2, 4, and 7, respectively, in the ischemia-reperfusion group. The histologically determined retinal thicknesses correlated with those obtained by tomographic images, but the histologic thicknesses were 9.5% to 18.5% thinner than those obtained by OCT. Fixation and dehydration of the histological specimens most likely caused tissue shrinkage.
Conclusions:
OCT can detect retinal changes quantitatively after ischemia-reperfusion injury, and the retinal thicknesses obtained from OCT images are probably a better measure of the true retinal thickness than those measured on histological sections.
