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Updated: Aug 18, 2026
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
[The methylation pattern of LRP15 gene in acute leukemia]
1Department of Hematology, PLA General Hospital, Beijing 100853, China. lipingdou@yahoo.com.cn
Objective:
To investigate the methylation status of LRP15 gene in acute leukemia (AL) and its role in tumorigenesis.
Methods:
73 cases of AL and 9 healthy subjects as well as COS7, K562 and HL60 cell lines were studied with methylation specific PCR (MSP).
Results:
LRP15 was not detected in all the samples. No LRP15 methylation was detected in COS7, but LRP15 was methylated in K562 and HL60. In nearly all of the French-American-British leukemia subtypes, we found that the frequency of LRP15 methylation was 71.2% (52/73) of AL and none in the 9 healthy subjects. The difference in mean methylation for LRP15 between these two kinds of samples is statistically significant (P < 0.05). Hypermethylation of the LRP15 gene was found in 57.1% (16/28) of the newly diagnosed AL and 83.3% of the relapsed AL respectively; this is also statistically significant (P < 0.05).
Conclusion:
LRP15 methylation change is a common abnormality in leukemia and LRP15 is postulated to be a tumor suppressor gene.
