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Updated: Aug 18, 2026

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
Published on: September 24, 2021
Properties of BK(Ca) channels in oral keratinocytes
1Institute of Oral Medicine, National Cheng Kung University Medical College, No. 1, University Road, Tainan 701, Taiwan.
Abstract:
Keratinocytes are important for epithelial antimicrobial barrier function. The activity of ion channels can affect the proliferation of keratinocytes. Little is known about Ca2+-activated K+ currents in these cells. Ion currents in normal human oral keratinocytes were characterized with a patch-clamp technique. In whole-cell configuration, depolarizing pulses evoked K+ outward currents (I(K)) in oral keratinocytes. Iberiotoxin (200 nM) and paxilline (1 microM) suppressed I(K); however, neither apamin (200 nM) nor 5-hydroxydecanoate (30 microM) had any effects on it. Caffeic acid phenethyl ester, a compound of honeybee propolis, increased I(K) with an EC50 value of 12.8 +/- 1.2 microM. In inside-out patches, a BK(Ca) channel was observed in keratinocytes, but not in oral squamous carcinoma (OCE-M1) cells. Caffeic acid phenethyl ester or cinnamyl-3,4-dihydroxy-alpha-cyanocinnamate applied to the intracellular surface of a detached patch increased BK(Ca)-channel activity. The results demonstrate that the properties of BK(Ca) channels in normal human oral keratinocytes are similar to those described in other types of cells. Caffeic acid derivatives can also stimulate BK(Ca)-channel activity directly.
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