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Related Experiment Videos

Interactions between immune-stimulating complexes (ISCOMs) and peritoneal mononuclear leucocytes.

D L Watson1, N A Watson, C Fossum

  • 1CSIRO Division of Animal Health, Armidale NSW, Australia.

Microbiology and Immunology
|January 1, 1992
PubMed
Summary
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Immune-stimulating complexes (ISCOMs) containing influenza virus antigens show enhanced cell membrane interaction and uptake compared to micelles. PR8 ISCOMs significantly boosted immune cell Ia expression in mice.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Influenza virus envelope glycoproteins are key targets for immune responses.
  • Delivery systems like immune-stimulating complexes (ISCOMs) and micelles are used to present viral antigens.
  • Understanding the interaction of these delivery systems with cell membranes is crucial for vaccine development.

Purpose of the Study:

  • To compare the cellular interaction and immune-stimulating properties of influenza virus-derived ISCOMs and micelles.
  • To investigate the effect of PR8 ISCOMs on the expression of membrane Ia molecules on immune cells.

Main Methods:

  • Preparation of immune-stimulating complexes (ISCOMs) and micelles from human influenza virus (PR8) envelope glycoproteins and matrix proteins.
  • Electron microscopy to visualize the interaction of ISCOMs and micelles with cell membranes.

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  • Intraperitoneal immunization of mice and subsequent analysis of peritoneal mononuclear leukocytes for Ia expression.

    • Main Results:

    • Electron microscopy revealed that ISCOMs exhibit a strong affinity for cell membranes and promote rapid internalization.
    • Micelles did not show the same level of membrane interaction or internalization as ISCOMs.
    • PR8 ISCOMs, but not matrix or micelles alone, significantly increased the expression of membrane Ia on peritoneal mononuclear leukocytes 24 hours post-immunization.

    Conclusions:

    • Immune-stimulating complexes (ISCOMs) demonstrate superior cell membrane affinity and internalization compared to micelles.
    • PR8 ISCOMs effectively enhance the expression of major histocompatibility complex class II (Ia) molecules on immune cells, suggesting potent immunomodulatory activity.
    • ISCOMs represent a promising platform for enhancing immune responses against influenza virus.