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Updated: Aug 16, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
[Population studies of Exophiala jeanselmeis by sequence analysis in the ribosomal DNA]
Dong-ming Li1, Ruo-yu Li, Xiao-hong Wang
1Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Objective:
To study the populations of Exophiala jeanselmei strains from diverse sources using rDNA sequences.
Methods:
Tested strains included 20 isolates of Exophiala jeanselmei (type culture, reference culture, clinical and environmental isolates), 8 isolates of E. moniliae, 1 isolate of Exophiala werneckii, respectively. DNA was isolated with boiled-iced water method. Ribosomal DNA and its internal transcribed spacer regions were amplified and analyzed with sequencing.
Results:
The ribosomal genes of E. jeanselmei varied greatly. Twenty strains of this species were divided into 7 types. The genetic distances of some strains in this species were quite long.
Conclusion:
The ribosomal RNA genes and their internal transcript sequences vary more widely than those of the other species in this genus.
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