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Storage hexamer utilization in Manduca sexta.

William H Telfer1, M L Pan

  • 1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018, USA. wtelfer@sas.upenn.edu

Journal of Insect Science (Online)
|April 21, 2005
PubMed
Summary
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Adult female insects retain significant high methionine proteins (M-MtH and V-MtH) after metamorphosis for egg formation, unlike males. These reserves are maintained through larger female pupal stores and differential utilization during development.

Area of Science:

  • Biochemistry
  • Insect Physiology
  • Molecular Biology

Background:

  • Insects store nutrient reserves as 500-kDa hexamerins in hemolymph and fat bodies for metamorphosis.
  • Lepidoptera utilize arylphorin (ArH) and high methionine proteins (M-MtH, V-MtH) for this purpose.

Purpose of the Study:

  • To investigate the factors contributing to high methionine protein reserves in adult female Manduca sexta.
  • To understand the differential utilization and storage of hexamerins between male and female adult insects.

Main Methods:

  • Quantification of hexamerin reserves in adult Manduca sexta at six days post-eclosion.
  • Centrifugation of hemolymph homogenates to separate soluble and particle-associated hexamerin fractions.
  • Comparison of hexamerin storage and utilization profiles between sexes.

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Main Results:

  • Adult males consumed over 99% of pupal hexamerins, while females retained over 25% of high methionine proteins.
  • Female pupal stores of methionine proteins were 1.67 times larger than males.
  • Particle-associated hexamerins constituted over half of the reserves in post-eclosion females, with reserves doubling post-eclosion.

Conclusions:

  • Female Manduca sexta prioritize retaining high methionine proteins for egg formation.
  • Differential utilization of soluble versus particle-associated hexamerins contributes to female nutrient reserves.
  • Adult females may resume synthesis of V-MtH and M-MtH post-eclosion.