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D: -Mannitol formation from D: -glucose in a whole-cell biotransformation with recombinant Escherichia coli
Björn Kaup1, Stephanie Bringer-Meyer, Hermann Sahm
1Karl-Winnacker-Institut, Bioverfahrenstechnik, DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt, Germany.
Abstract:
Recently, we reported on the construction of a whole-cell biotransformation system in Escherichia coli for the production of D: -mannitol from D: -fructose. Supplementation of this strain with extracellular glucose isomerase resulted in the formation of 800 mM D: -mannitol from 1,000 mM D: -glucose. Co-expression of the xylA gene of E. coli in the biotransformation strain resulted in a D: -mannitol concentration of 420 mM from 1,000 mM D: -glucose. This is the first example of conversion of D: -glucose to D: -mannitol with direct coupling of a glucose isomerase to the biotransformation system.
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