Related Experiment Video
Updated: Aug 18, 2026
Establishment of Rat Models Mimicking Gender-affirming Hormone Therapies
Published on: January 10, 2025
Distribution of thyrostimulin in the rat: an immunohistochemical study
Chang Li1, Yoshifumi Hirooka, Satoshi Habu
1Department of Laboratory Medicine, Aichi Medical University School of Medicine, Nagakute, Aichi, Aichi, Japan.
Objective:
To identify the distribution of thyrostimulin, a heterodimer of glycoprotein hormone subunits (A2 and B5) by immunohistochemistry in the rat tissues using specific antipeptide anti-serum which we recently produced.
Method:
Anti-thyrostimulin antibody was raised in New Zealand white rabbits immunized with a conjugate of synthetic A2 or B5 with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method.
Results:
Thyrostimulin immunoreactivity was visualized in the anterior pituitary, central nervous system, adrenal gland, stomach, duodenum, pancreas and testis. When using antiserum pre-incubated with synthetic peptides or rat pituitary homogenate which contains thyrostimulin peptide, no significant stain of the pituitary was detected.
Conclusion:
These findings suggest that thyrostimulin is widely distributed and that the method used is valuable in studying the distribution of thyrostimulin in rats.
