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Updated: Aug 18, 2026
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
Effect of leptin on proliferative activity and vascular endothelial growth factor (VEGF) secretion from cultured
Beata Misztal-Dethloff1, Henryk Stepien, Jan Komorowski
1Institute of Endocrinology, Medical University of Lodz, 91-425 Lodz, Poland.
Objective:
The aim of this study was to check if leptin influences the proliferative activity and vascular endothelial grofth factor (VEGF) release from cultured mouse endothelial cells in vitro.
Methods:
The murine cell line HECa10 obtained from endothelial cells of mouse peripheral lymph nodes immortalised by transfection of plasmid with the gene for the large T antigen of Simian virus 40 was used in the experiments. The proliferative activity of HECa10 cells was studied by Mosmann and VEGF release by ELISA methods.
Results:
Murine leptin in concentrations from 5 to 25 ng/ml stimulated the proliferative activity of 72 h endothelial cell cultures and in concentrations from 0.5 to 25 ng/ml augmented the release of VEGF into supernatants of 24 h and 72 h cultured cells.
Conclusion:
Leptin stimulated proliferation and VEGF secretion of endothelial cells in vitro.
