Distribution of the limbic system-associated membrane protein (LAMP) in pigeon forebrain and midbrain

The limbic system-associated membrane protein (LAMP) is an adhesion molecule involved in specifying regional identity during development, and it is enriched in the neuropil of limbic brain regions in mammals but also found in some somatic structures. Although originally identified in rat, LAMP is present in diverse species, including avians. In this study, we used immunolabeling with a monoclonal antibody against rat LAMP to examine the distribution of LAMP in pigeon forebrain and midbrain. LAMP immunolabeling was prominent in many telencephalic regions previously noted as limbic in birds. These regions include the hippocampal complex, the medial nidopallium, and the ventromedial arcopallium. Subpallial targets of these pallial regions were also enriched in LAMP, such as the medial-most medial striatum. Whereas some telencephalic areas that have not been regarded as limbic were also LAMP-rich (e.g., the hyperpallium intercalatum and densocellulare of the Wulst, the mesopallium, and the intrapeduncular nucleus), most nonlimbic telencephalic areas were LAMP-poor (e.g., field L, the lateral nidopallium, and somatic basal ganglia). Similarly, in the diencephalon and midbrain, prominent LAMP labeling was observed in such limbic areas as the dorsomedial thalamus, the hypothalamus, the ventral tegmental area, and the central midbrain gray, as well as in a few nonlimbic areas such as nucleus rotundus, the shell of the nucleus pretectalis, the superficial tectum, and the parvocellular isthmic nucleus. Thus, as in mammals, LAMP in birds appears to be enriched in most known forebrain and midbrain limbic structures but is present as well in some somatic structures.