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Neural cell adhesion molecule in aged mouse muscle.

H Kobayashi1, N Robbins, U Rutishauser

  • 1Department of Genetics, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4901.

Neuroscience
|January 1, 1992
PubMed
Summary

Aging increases neural cell adhesion molecule (NCAM) expression in specific mouse muscles, suggesting its role in age-related motor nerve terminal instability. This study investigated NCAM changes in aging neuromuscular junctions.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Aging Research

Background:

  • Neuromuscular junctions undergo age-related changes, including synaptic remodeling.
  • Neural cell adhesion molecule (NCAM) is crucial for neuronal development and function.

Purpose of the Study:

  • To investigate age-related changes in NCAM expression in skeletal muscles.
  • To correlate NCAM expression with age-related neuromuscular morphology in mice.

Main Methods:

  • Comparative analysis of NCAM expression in endplate and non-endplate regions of skeletal muscles from mature and old mice.
  • Densitometry of immunoblots and immunocytochemical studies were employed.
  • Three muscles (soleus, sternomastoid, diaphragm) with varying degrees of age-related remodeling were examined.

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Main Results:

  • Increased expression of the 140,000 mol. wt. NCAM form in the endplate regions of old mice soleus and sternomastoid muscles.
  • NCAM expression remained unchanged with age in the diaphragm muscle.
  • A proteolytic fragment of NCAM (70,000-80,000 mol. wt.) increased in all old muscles.
  • Increased NCAM-positive nerve fibers observed in the motor nerve of old sternomastoid muscles.

Conclusions:

  • Selective upregulation of NCAM in junctional regions of remodeling muscles in old mice suggests a role in motor nerve terminal instability.
  • Age-related neuromuscular remodeling is associated with specific alterations in NCAM expression.
  • Findings indicate NCAM may be implicated in the functional decline of neuromuscular junctions during aging.