Related Experiment Video
Updated: Aug 18, 2026
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Droplet-based micro-flow chemiluminescence system for in vivo glucose determination by microdialysis sampling
Wei Liu1, Zhujun Zhang, Haisheng Liu
1Department of Chemistry, Institute of Analytical Science, Southwest Normal University, Beibei, Chongqing 400715, PR China.
Abstract:
A micro-flow chemiluminescence (CL) system in vivo for glucose determination by the on-line microdialysis sampling is described in this paper. The micro-flow CL system uses discrete sample droplets, which formed at the tip of the capillary with the sampling volume of 4.5 microl. The sol-gel method is introduced to co-immobilize horseradish peroxidase (HRP) and glucose oxidase (GOD) on the inside surface of the micro-flow cell which was fabricated in polymethyl methacrylate (PMMA). The CL detection involved enzymatic oxidation of glucose to D-gluconic acid and H2O2, then H2O2 oxidizing luminol to produce CL in presence of HRP. The microdialysis probe was utilized for sampling in the rabbit blood; the sample throughput was 20 h(-1). The glucose level in blood of the rabbit was on-line monitored with good results.
