Related Experiment Video
Updated: Aug 18, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
Published on: April 16, 2017
Spatial resolution improvement of scanning microscopy based on thermal lens spectroscopy with a
Takuya Shimosaka1, Masakazu Izako, Katsumi Uchiyama
1Tokyo Metropolitan University, School of Engineering, Minamiosawa 1-1, Hachioji, Tokyo 192-0397, Japan. t-shimosaka@aist.go.jp
Abstract:
In scanning microscopy by total internal reflection with thermal lens spectroscopy, its spatial resolution depends on the distance between the sample and a converging lens, which corresponds to the objective lens in an ordinary optical microscope. It was found that the resolution was best when the signal induced by the thermal lens effect was maximum. The distance was precisely adjusted by monitoring the signal intensity, and the resolution became twice better than that previously reported. Using a shorter focal-length lens, a resolution of 1.9 microm was attained.
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Super-resolution Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

