Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NF-kappa B comes home.

Rupesh H Amin1, Mark S Schlissel

  • 1Department of Molecular and Cell Biology, Division of Immunology, University of California, Berekely, 94720, USA.

Immunity
|April 23, 2005
PubMed
Summary

Nuclear factor kappa B (NF-kappa B) regulates immunoglobulin kappa (Ig kappa) rearrangement by activating RAG expression. This occurs during receptor editing, a critical process in adaptive immunity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Support U.S. research during COVID-19.

Science (New York, N.Y.)·2020
Same author

A new data effort to inform career choices in biomedicine.

Science (New York, N.Y.)·2017
Same author

Dual Mechanism of <i>Rag</i> Gene Repression by c-Myb during Pre-B Cell Proliferation.

Molecular and cellular biology·2017
Same author

Pillars Article: Cell Type-Specific Chromatin Structure Determines the Targeting of V(D)J Recombinase Activity In Vitro. Cell. 1996. 85: 887-897.

Journal of immunology (Baltimore, Md. : 1950)·2015
Same author

The proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination.

PloS one·2015
Same author

Gfi1 and gfi1b repress rag transcription in plasmacytoid dendritic cells in vitro.

PloS one·2013

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Nuclear factor kappa B (NF-kappa B) is a transcription factor crucial for immune responses.
  • Its role in immunoglobulin kappa (Ig kappa) V(D)J recombination has been debated due to conflicting experimental evidence.
  • Previous studies indicated NF-kappa B binding sites were not essential for Ig kappa rearrangement.

Discussion:

  • Verkoczy et al. present new findings challenging the established understanding of NF-kappa B's function in Ig kappa rearrangement.
  • The study demonstrates that NF-kappa B plays a regulatory role, not by direct DNA binding at the enhancer, but by controlling the expression of key recombination machinery.
  • This mechanism is particularly relevant during receptor editing, a process where B cells modify their antigen receptors.

Key Insights:

  • NF-kappa B activation is essential for inducing RAG expression during Ig kappa V(D)J recombination.
  • Receptor editing, a critical B cell tolerance mechanism, is directly regulated by NF-kappa B-mediated RAG induction.
  • This provides a novel perspective on how the adaptive immune system maintains self-tolerance.

Outlook:

  • Further investigation into the precise signaling pathways linking NF-kappa B to RAG expression is warranted.
  • Understanding this regulatory axis could reveal new therapeutic targets for autoimmune diseases and B cell malignancies.
  • This work highlights the complex, context-dependent roles of transcription factors in adaptive immunity.

Related Experiment Videos