Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcripts from a human primordial follicle cDNA library.

Maria D Serafica1, Tetsuya Goto, Alan O Trounson

  • 1MISCL (Monash Immunology and Stem Cell Laboratories), Monash University, Wellington Road, Clayton, Victoria, 3800 Australia. maria.serafica@med.monash.edu.au

Human Reproduction (Oxford, England)
|April 23, 2005
PubMed
Summary

This study reveals key genes and pathways involved in early human oogenesis, providing a molecular blueprint of primordial follicles (PFs). Understanding these factors is crucial for oogenesis research and potential fertility treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Energy-dependent cortical injury thresholds in high-frequency transcortical electrical stimulation: a biophysical study in a rat model.

Experimental brain research·2026
Same author

CAR-NK Engineering to Overcome TME Barriers.

Cells·2026
Same author

Engineering TAG-72 and CD30 CAR-T Cells for T Cell Malignancies.

Immunological investigations·2025
Same author

CRISPR-Cas9 knockout of DGKα/ζ improves the anti-tumor activities of TAG-72 CAR-T cells in ovarian cancer.

Molecular therapy. Oncology·2025
Same author

Profiling chronic migraine patients according to clinical characteristics: a cluster analysis approach.

Frontiers in neurology·2025
Same author

Treatment Patterns, Characteristics, and Probable Acute Medication Overprescription Among Patients With Migraine in Japan: A Retrospective Cross-Sectional and Longitudinal Analysis of Health Insurance Claims Data.

Cureus·2025

Area of Science:

  • Reproductive Biology
  • Genomics
  • Molecular Biology

Background:

  • Human primordial follicles (PFs) represent the earliest stage of oogenesis.
  • The molecular mechanisms governing PF growth and recruitment are largely unknown.

Purpose of the Study:

  • To establish a gene resource for human PFs.
  • To create a molecular blueprint of the human PF.

Main Methods:

  • A cDNA library was constructed from 50 isolated human PFs.
  • Phagemid vector pTriplEx2 was utilized for library construction.
  • Bioinformatics analysis was performed on sequenced clones.

Main Results:

  • 46.67% of clones were known genes, 29.48% were uncharacterized.

Related Experiment Videos

  • Identified genes involved in mitochondrial function, transcription, translation, cell cycle, and apoptosis.
  • Detected key signaling pathways including MAPK, Wnt, and Notch.

    • Conclusions:

    • Abundant retroviral elements and transcriptional repressors may maintain the PF stage.
    • A molecular blueprint of human PFs was generated.
    • A human PF cDNA array could facilitate future research on oogenesis.