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Updated: Aug 1, 2026

06:45
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Published on: February 28, 2019
Comment on "Force-clamp spectroscopy monitors the folding trajectory of a single protein"
Robert B Best1, Gerhard Hummer
1Laboratory of Chemical Physics, National Institute of Diabetes andDigestive and Kidney Diseases, National Institutes of Health, Building 5, Bethesda MD 20892-0520, USA.
Abstract
No abstract available in PubMed .
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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

