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Updated: Aug 9, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
The interleukin-6--174 single nucleotide polymorphism: cervical protein production and the risk of preterm delivery
Whitney E Jamie1, Rodney K Edwards, Ronald J Ferguson
1Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, FL, USA.
Objective:
The purpose of this study was to determine the associations between preterm delivery (PTD), cervical fluid interleukin-6 (IL-6) concentration, and the single nucleotide polymorphism at position -174 in the IL-6 gene.
Study Design:
Cervical fluid samples were obtained from women 23 to 32 weeks' gestation with symptoms of preterm labor. Concentrations of IL-6 were determined by enzyme-linked immunosorbent assay (ELISA). IL-6 genotyping was performed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
Results:
One hundred thirty-seven women were enrolled, and complete information was available for 126. Cervical fluid IL-6 concentrations were not elevated in women destined to have a spontaneous PTD ( P = .86). IL-6 -174 genotype was not associated with PTD ( P = .62) or cervical fluid IL-6 concentration ( P = .36). Neonatal IL-6-174 genotype was not associated with PTD or IL-6 concentration.
Conclusion:
Cervical fluid concentrations of IL-6 were not elevated in symptomatic women destined to have a spontaneous PTD. The presence of maternal IL-6 -174C was not associated with cervical fluid concentration of IL-6 or risk of PTD.
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