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Updated: Aug 18, 2026
A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
[Screening and identification of a strain for hydroxylation of nicotinic acid]
Wei-hong Lu1, Li Xu, Yi-jun Dai
1Jiangsu Key Laboratory for Bioresource technology, NNU Laboratory of Microbiology and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. luweihong168@sohu.com
Abstract:
Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.
