Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A flexible array format for large-scale, rapid blood group DNA typing.

Ghazala Hashmi1, Tasmia Shariff, Michael Seul

  • 1BioArray Solutions Ltd, Warren, New Jersey, USA.

Transfusion
|April 26, 2005
PubMed
Summary

Microarray technology offers a feasible alternative to traditional hemagglutination for blood group typing. This DNA analysis method is cost-effective and efficient for identifying blood group alleles and discovering new ones in diverse donor populations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Survey of national and regional rare donor programmes regarding Immunoglobulin A deficiency.

Vox sanguinis·2026
Same author

Identification and structural analysis of a novel FUT1 c.789C>A variant and previously reported para-Bombay alleles using an α2FucT1 structural model.

Transfusion·2026
Same author

International Society of Blood Transfusion Working Party on Red Cell Immunogenetics and Blood Group Terminology Report of Gothenburg, Barcelona and four virtual business meetings: Update on blood group systems.

Vox sanguinis·2025
Same author

A novel RHD allele combining partial D (c.455A>C) and weak D (c.1154G>C) variants.

Transfusion·2025
Same author

Investigation of unexplained reactivity: antibody of unknown specificity (AUS).

Immunohematology·2025
Same author

Weak and partial D phenotyping: a comparison study between molecular and serologic results.

Immunohematology·2024

Area of Science:

  • Genetics
  • Immunology
  • Biotechnology

Background:

  • Current blood group antigen typing relies on hemagglutination, which is costly, labor-intensive, and faces reagent limitations.
  • The need for more efficient and scalable blood typing methods is critical for transfusion medicine and population genetics.

Purpose of the Study:

  • To evaluate the feasibility of using large-scale DNA analysis on microarrays as a substitute for conventional blood group typing.
  • To assess the performance of bead array technology for identifying red blood cell antigen alleles.

Main Methods:

  • DNA was extracted from blood samples and analyzed for blood group alleles using bead array (BeadChip) and polymerase chain reaction (PCR)-based assays.
  • A prototype BeadChip (BLOOD-1) with single-nucleotide polymorphisms (SNPs) for various blood group alleles was validated against serological data.

Related Experiment Videos

  • An expanded BeadChip with 18 SNPs was used to analyze DNA from diverse ethnic donor cohorts.

    • Main Results:

    • BeadChip analysis results demonstrated high concordance with established PCR and hemagglutination methods for blood group typing.
    • Allele frequencies in different ethnic panels aligned with expected ranges, indicating the reliability of the microarray approach.
    • The study successfully identified two novel DO alleles within the tested donor populations.

    Conclusions:

    • Microarray-based DNA analysis is a viable and effective method for blood group typing.
    • This technology facilitates the identification of known and new alleles in large donor cohorts, offering a scalable alternative to traditional methods.