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Related Experiment Videos

Immunostimulatory human urinary protein.

E Fontan1, H Saklani-Jusforgues, R M Fauve

  • 1Unité d'Immunophysiologie cellulaire, Institut Pasteur, Paris, France.

Proceedings of the National Academy of Sciences of the United States of America
|May 15, 1992
PubMed
Summary
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A novel human glycoprotein, HGP.43, derived from urine, effectively protects mice against lethal Listeria monocytogenes infection. This discovery offers a new avenue for developing treatments against bacterial infections.

Area of Science:

  • Immunology
  • Microbiology
  • Biochemistry

Background:

  • A previously identified mouse granuloma protein demonstrated protective effects against Listeria monocytogenes.
  • This study investigates a similar protective factor in human urine.

Purpose of the Study:

  • To identify and characterize a human protein with protective activity against Listeria monocytogenes.
  • To evaluate the efficacy of this human protein in preventing lethal bacterial infection.

Main Methods:

  • Polyclonal and monoclonal antibodies were used to identify and inhibit the protective factor in human urine.
  • Purification of the active component yielded a 43 kDa glycoprotein (HGP.43).
  • Efficacy was tested in mouse models, including severe combined immunodeficient (SCID) mice, against lethal Listeria monocytogenes challenge.

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Main Results:

  • A human urinary fraction containing HGP.43 conferred full protection against lethal Listeria monocytogenes infection in mice.
  • Murine monoclonal antibodies neutralized this protective activity.
  • Intravenous injection of purified HGP.43 (250 µg/kg) protected mice when administered up to 8 hours post-infection.
  • HGP.43 also induced cytostatic effects on inflammatory murine macrophages against Lewis carcinoma cells.

Conclusions:

  • Human glycoprotein 43 kDa (HGP.43) is a potent protective agent against Listeria monocytogenes infection in mice, independent of T and B cell immunity.
  • HGP.43 demonstrates potential as a therapeutic agent for bacterial infections and warrants further investigation for immunomodulatory properties.