Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Environment affects amino acid preference for secondary structure.

L Zhong1, W C Johnson

  • 1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.

Proceedings of the National Academy of Sciences of the United States of America
|May 25, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Uterine epithelioid leiomyosarcoma with PGR::NR4A3 fusion gene: report of a case].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

Retraction Note: Clinical significance of serum hepcidin-25 levels in predicting invasive fungal disease in patients after transplantation.

European review for medical and pharmacological sciences·2026
Same author

[The influence of two-way referral model on treatment and prognosis of patients with chronic heart failure].

Zhonghua xin xue guan bing za zhi·2025
Same author

[Status analysis of standard methods for detecting pesticide in the air of workplace in China].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

Pretreatment <sup>18</sup>F-FDG PET/CT in predicting the survival of patients with hepatic neuroendocrine tumors.

Revista espanola de medicina nuclear e imagen molecular·2025
Same author

[Epidemiological characteristics and influencing factors of diabetes and pre-diabetes among adult residents in Hainan Province].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025

Amino acid sequences can form different protein structures based on their environment. This study shows that the surrounding solvent system influences whether alpha-helical or beta-strand structures form, proving environment is key.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Science

Background:

  • Amino acid sequences are often used to predict protein secondary structure.
  • Computational methods predict alpha-helical structures for certain sequences based on amino acid preferences.
  • Experimental evidence is needed to validate these predictive models.

Purpose of the Study:

  • To investigate the influence of environmental factors on protein secondary structure formation.
  • To experimentally determine the secondary structure of synthesized amino acid sequences.
  • To challenge the accuracy of predicting secondary structure solely from amino acid sequence.

Main Methods:

  • Synthesis of three specific amino acid sequences.
  • X-ray diffraction analysis of the synthesized proteins.

Related Experiment Videos

  • Experimentation with various solvent systems to alter the environment.

    • Main Results:

    • Synthesized sequences predicted to be alpha-helical were found to be primarily beta-strand via x-ray diffraction.
    • Specific solvent systems allowed the recovery of the predicted alpha-helical structure.
    • Other solvent systems, mimicking a protein's interior, induced beta-strand formation.

    Conclusions:

    • Environmental conditions significantly dictate the secondary structure adopted by an amino acid sequence.
    • Predictive models relying exclusively on amino acid sequence for secondary structure prediction are inherently limited.
    • Experimental validation across diverse environments is crucial for understanding protein folding.