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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Immunogenicity of engineered antibodies

William Ying Khee Hwang1, Jefferson Foote

  • 1Singapore Health Services, Singapore General Hospital, Singapore.

Methods (San Diego, Calif.)
|April 26, 2005
PubMed

Administration of a therapeutic antibody can lead to an anti-antibody response (AAR). Much effort has been applied to engineering antibodies with as little as possible non-human structure to minimize such responses. Here, we review reported AAR to murine, mouse-human chimeric, and humanized antibodies. Replacement of mouse immunoglobulin constant regions with human ones effects the largest immunogenicity reduction. Humanization of variable domains effects a further decrease.

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